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Pratlong, M.; Haguenauer, A.; Chenesseau, S.; Brener, K.; Mitta, Guillaume; Toulza, E.; Bonabaud, M.; Rialle, S.; Aurelle, D.; Pontarotti, P.. |
dSexual reproduction is widespread among eukaryotes, and the sex-determining processes vary greatly among species. While genetic sex determination (GSD) has been intensively described in bilaterian species, no example has yet been recorded among non-bilaterians. However, the quasi-ubiquitous repartition of GSD among multicellular species suggests that similar evolutionary forces can promote this system, and that these forces could occur also in non-bilaterians. Studying sex determination across the range of Metazoan diversity is indeed important to understand better the evolution of this mechanism and its lability. We tested the existence of sexlinked genes in the gonochoric red coral (Corallium rubrum, Cnidaria) using restriction site-associated DNA... |
Tipo: Text |
Palavras-chave: Genetic sex determination; Corallium rubrum; RAD-sequencing; Cnidaria. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00382/49367/49769.pdf |
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Pivotto, I. D.; Nerini, D.; Masmoudi, M.; Kara, H.; Chaoui, L.; Aurelle, D.. |
Climate change has a strong impact on marine ecosystems, including temperate species. Analysing the diversity of thermotolerance levels within species along with their genetic structure enables a better understanding of their potential response to climate change. We performed this integrative study on the Mediterranean octocoral Eunicella cavolini, with samples from different depths and by means of a common garden experiment. This species does not host photosynthetic Symbiodinium, enabling us to focus on the cnidarian response. We compared the thermotolerance of individuals from 20 m and 40 m depths from the same site and with replicates from the same colony. On the basis of an innovative statistical analysis of necrosis kinetics and risk, we demonstrated... |
Tipo: Text |
Palavras-chave: Climate change; Adaptation; Acclimatization; Eunicella cavolini; Mediterranean sea; Population genetics. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00371/48264/48637.pdf |
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Gerard, K.; Guilloton, E.; Arnaud-haond, Sophie; Aurelle, D.; Bastrop, R.; Chevaldonne, P.; Derycke, S.; Hanel, R.; Lapegue, Sylvie; Lejeusne, C.; Mousset, Sylvain; Ramsak, A.; Remerie, T.; Viard, Frederique; Feral, Jean-pierre; Chenuil, A.. |
Exon Primed Intron Crossing (EPIC) markers providemolecular tools that are susceptible to be variable within specieswhile remaining amplifiable by PCR using potentially universal primers. In this studywe tested the possibility of obtaining PCR products from 50 EPIC markers on 23 species belonging to seven different phyla (Porifera, Cnidaria, Arthropoda, Nematoda, Mollusca, Annelida, Echinodermata) using 70 new primer pairs. A previous study had identified and tested those loci in a dozen species, including another phylum, Urochordata (Chenuil et al., 2010). Results were contrasted among species. The best results were achieved with the oyster (Mollusca) where 28 loci provided amplicons susceptible to contain an intron according to their size. This was... |
Tipo: Text |
Palavras-chave: Universal primers; Alternative barcoding; Non-model species; Genetic marker; Intron. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00166/27775/25969.pdf |
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Pratlong, M.; Haguenauer, A.; Chabrol, O.; Klopp, C.; Pontarotti, P.; Aurelle, D.. |
The question of species survival and evolution in heterogeneous environments has long been a subject for study. Indeed, it is often difficult to identify the molecular basis of adaptation to contrasted environments, and nongenetic effects increase the difficulty to disentangle fixed effects, such as genetic adaptation, from variable effects, such as individual phenotypic plasticity, in adaptation. Nevertheless, this question is also of great importance for understanding the evolution of species in a context of climate change. The red coral (Corallium rubrum) lives in the Mediterranean Sea, where at depths ranging from 5 to 600m, it meets very contrasted thermal conditions. The shallowest populations of this species suffered from mortality events linked... |
Tipo: Text |
Palavras-chave: Cnidarians; Gene expression; Local adaptation; Octocoral polymorphism; Transcriptome. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00371/48257/48661.pdf |
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